iCycler iQ™ Real-Time PCR Detection System Instruction . - Bio-Rad

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iCycler iQ Real-Time PCR Detection System Instruction Manual Catalog Number 170-8740 For Technical Service Call Your Local Bio-Rad Office or in the U.S. Call 1-800-4BIORAD (1-800-424-6723)

• Page 2 and 3: Safety Information Important: Read
• Page 4 and 5: 5.4.1 Well Factors .
• Page 7 and 8: Section 1 Introduction The Polymera
• Page 9 and 10: Excitation Filters Emission Filters
• Page 11 and 12: 3. To access the excitation filter
• Page 13 and 14: 3. Prepare the experimental PCR rea
• Page 15 and 16: EXPERIMENTAL PLATE FACTORS (Dynamic
• Page 17 and 18: Section 3 Introduction to the iCycl
• Page 19 and 20: 3.1.4 The Data Analysis Module This
• Page 21 and 22: Section 4 The Library Module From t
• Page 23 and 24: Fig. 4.2. Library / View Plate Setu
• Page 25 and 26: 4.4 View Post-Run Data Window The V
• Page 27 and 28: Section 5 The Workshop Module The W
• Page 29 and 30: 4. If you want to add any protocol
• Page 31 and 32: • Gradient. Two new columns appea
• Page 33 and 34: • Step: an individual temperature
• Page 35 and 36: Fig. 5.6. Protocol options. A. Infi
• Page 37 and 38: Melt curve analysis applications ar
• Page 39 and 40: A time increment or decrement may b
• Page 41 and 42: Fig. 5.8. Workshop / Edit Plate Set
• Page 43 and 44: Programming Note: It is permissible
• Page 45 and 46: 8. Choose one of the filter wheel s
• Page 47 and 48: • Click the top corner to select
• Page 49 and 50: In these cases, icons will appear i
• Page 51 and 52: Alternatively, you can specify the
• Page 53 and 54: • To change standard quantities o
• Page 55 and 56: Note: You may save plate setup and
• Page 57 and 58: Procedure: 1. Confirm that the desi
• Page 59 and 60: B. Using the Experimental Plate for
• Page 61 and 62: After you click Begin Run, the iCyc
• Page 63 and 64: B. Running a Pure Dye Calibration P
• Page 65 and 66: Section 6 The Run-Time Central Modu
• Page 67 and 68: B. Skip to Next Cycle • Click Ski
• Page 69 and 70: Fig. 6.4. Imaging Services Window.
• Page 71 and 72: 6. Click Make an Expose. An image o
• Page 73 and 74: Section 7 Data Analysis Module Intr
• Page 75 and 76: To edit a well description 1. Click
• Page 77 and 78: Fig. 7.6. Modified Well Data Spread
• Page 79 and 80: 7. Once all adjustments are made, s
• Page 81 and 82: Fig. 7.12. Set Data Analysis window
• Page 83 and 84: The weighted mean is determined by
• Page 85 and 86: Fig. 7.18. Well selection for Defin
• Page 87 and 88: • PCR Baseline Subtracted. In ord
• Page 89 and 90: Fig. 7.26. Threshold Cycle Calculat
• Page 91 and 92: Fig. 7.28. Standard Curve Window.
• Page 93 and 94: The second is of the negative first
• Page 95 and 96: Fig. 7.34. Melting Curve Plot Sprea
• Page 97 and 98: 3. Click Apply Changes to Melt Peak
• Page 99 and 100: 7.5.2 Allelic Discrimination Plot T
• Page 101 and 102: 7.5.3 Allelic Discrimination Plot C
• Page 103 and 104: Fig. 7.42. Manual Call Selection. 2
• Page 105 and 106: Section 8 Care and Maintenance 8.1
• Page 107 and 108: Appendix B Minimum Computer Specifi
• Page 109 and 110: Appendix D Product Information Cata
• Page 111 and 112: Cause: You have created a thermal p
• Page 113 and 114: Cause: This alert is similar to the
• Page 115 and 116: Cause: The lid has been opened whil
• Page 117 and 118: Cause: One or more wells on the exp
• Page 119 and 120: Appendix F Hardware Error Messages
• Page 121 and 122: Appendix G Description of iCycler i
• Page 123 and 124: A. The Background Subtracted graph
• Page 125 and 126: Fig. G.4. Determining Threshold Lev
• Page 127 and 128: Appendix H Uploading New Versions o
• Page 129 and 130: The second RME file would be approp
• Page 131 and 132: Appendix K Melt Curve Functionality
• Page 133 and 134: A molecular beacon and its target a
• Page 135 and 136: Section 2 Interpretation of Data 2.
• Page 137 and 138: Two peaks are identified in the mel
• Page 139 and 140: of a desired dilution series. By de
• Page 141 and 142: 2.3 Single Nucleotide Polymorphism
• Page 143 and 144: Appendix L Filter Wheel Setup and F
• Page 145 and 146: Appendix M New features in v.3.1 Op